Abstract

Gamma radiation induction for mutation is one method of plant breeding that increases genetic variability. By using gamma radiation to create genetic diversity in plants, it helps in developing traits such as disease resistance, adaptation to environmental changes, or increased yield. The mutations induced by gamma radiation lead to genetic variation, which has the potential to develop plant varieties with desired characteristics. The objective of this experiment was to evaluate the effects of gamma radiation on the germination of KU80 Ninlakan rice seeds. In the experiment, rice seeds were subjected to acute gamma radiation at doses of 100, 200, 300, 400, 500, 600, 700, 800, 900, and 1,000 Gray, and the effects on seed germination were measured. The results showed that higher radiation doses led to a decrease in seed germination. Rice seeds that were exposed to high doses of radiation exhibited a significantly lower germination rate. When considering the germination rate at 50 percent, or the LD50 value, the results indicated that the optimal radiation dose for inducing mutations was 380 Gray. This dose did not drastically reduce germination and could still contribute to the development of improved rice varieties with desired traits. The obtained data will be used in the breeding process through mutation induction.